Rapport d'Analyse Scientifique - Soresco Lab<\/title>\r\n <link rel=\"preconnect\" href=\"https:\/\/fonts.googleapis.com\">\r\n <link rel=\"preconnect\" href=\"https:\/\/fonts.gstatic.com\" crossorigin>\r\n <link\r\n href=\"https:\/\/fonts.googleapis.com\/css2?family=Inter:wght@300;400;500;600&family=Georgia:ital,wght@0,400;1,400&display=swap\"\r\n rel=\"stylesheet\">\r\n <style>\r\n <\/head><body>< !-- FULL WIDTH WRAPPER FOR WORDPRESS --><div style=\"width: 100vw; margin-left: calc(-50vw + 50%); position: relative; overflow-x: hidden; background: #fff;\">h1,\r\n h2,\r\n h3,\r\n h4,\r\n h5 {\r\n font-family: 'Georgia', serif;\r\n color: #3A3930;\r\n font-weight: 400;\r\n }\r\n\r\n .container {\r\n max-width: 95%;\r\n \/* Increased from 1000px for full width feel *\/\r\n margin: 0 auto;\r\n padding: 0 80px;\r\n \/* Extra padding left and right *\/\r\n }\r\n\r\n \/* HEADER *\/\r\n header {\r\n background-color: #fff;\r\n padding: 40px 0;\r\n border-bottom: 2px solid #F0EFE9;\r\n }\r\n\r\n .report-meta {\r\n font-size: 16px;\r\n \/* Increased meta font size *\/\r\n color: #6E6C55;\r\n display: grid;\r\n grid-template-columns: repeat(auto-fit, minmax(200px, 1fr));\r\n gap: 20px;\r\n margin-bottom: 40px;\r\n background: #FCFCFA;\r\n padding: 20px;\r\n border: 1px solid #EAE8DC;\r\n border-radius: 4px;\r\n }\r\n\r\n .report-meta strong {\r\n display: block;\r\n color: #3A3930;\r\n font-weight: 600;\r\n margin-bottom: 5px;\r\n }\r\n\r\n \/* TABLES *\/\r\n .scientific-table {\r\n width: 100%;\r\n border-collapse: collapse;\r\n font-size: 16px;\r\n \/* Increased table font size *\/\r\n margin-bottom: 40px;\r\n box-shadow: 0 2px 10px rgba(0, 0, 0, 0.03);\r\n }\r\n\r\n .scientific-table th,\r\n .scientific-table td {\r\n padding: 12px 15px;\r\n text-align: left;\r\n border: 1px solid #EAE8DC;\r\n }\r\n\r\n .scientific-table th {\r\n background-color: #F4F2E9;\r\n color: #3A3930;\r\n font-weight: 600;\r\n position: sticky;\r\n top: 0;\r\n }\r\n\r\n .scientific-table tr:nth-child(even) {\r\n background-color: #FCFCFA;\r\n }\r\n\r\n .scientific-table tr:hover {\r\n background-color: #F9F8F4;\r\n }\r\n\r\n .highlight-row {\r\n background-color: #FFFBE6 !important;\r\n }\r\n\r\n .section-separator {\r\n margin: 60px 0;\r\n height: 1px;\r\n background: #D4D1BC;\r\n opacity: 0.5;\r\n }\r\n\r\n \/* BIOLOGICAL STUDY *\/\r\n .bio-study-block {\r\n margin-bottom: 60px;\r\n }\r\n\r\n .protocol-box {\r\n background: #F9F8F4;\r\n padding: 30px;\r\n border-left: 4px solid #84825A;\r\n margin: 20px 0 30px;\r\n font-size: 16px;\r\n \/* Increased font size *\/\r\n }\r\n\r\n .results-box {\r\n background: #ffffff;\r\n border: 1px solid #EFECE1;\r\n padding: 30px;\r\n border-radius: 8px;\r\n }\r\n\r\n .result-highlight {\r\n font-size: 18px;\r\n color: #84825A;\r\n font-family: 'Georgia', serif;\r\n margin-bottom: 10px;\r\n display: block;\r\n }\r\n\r\n ul.dashed {\r\n list-style: none;\r\n padding-left: 0;\r\n }\r\n\r\n ul.dashed li {\r\n position: relative;\r\n padding-left: 20px;\r\n margin-bottom: 10px;\r\n }\r\n\r\n ul.dashed li::before {\r\n content: \"-\";\r\n position: absolute;\r\n left: 0;\r\n color: #84825A;\r\n }\r\n\r\n .print-only {\r\n display: none;\r\n }\r\n\r\n @media print {\r\n .print-only {\r\n display: block;\r\n }\r\n\r\n .no-print {\r\n display: none;\r\n }\r\n\r\n body {\r\n font-size: 12px;\r\n }\r\n\r\n .container {\r\n max-width: 100%;\r\n padding: 0;\r\n }\r\n }\r\n <\/style>\r\n<\/head>\r\n\r\n\r\n\r\n<header>\r\n <div class=\"container\">\r\n <div style=\"text-align: center; margin-bottom: 40px;\">\r\n <h4 style=\"margin: 0; font-size: 14px; letter-spacing: 0.2em; text-transform: uppercase; color: #84825A;\">\r\n Centre de Biotechnologie de Borj-Cedria (CBBC)<\/h4>\r\n <div style=\"font-size: 12px; margin-top: 5px; opacity: 0.7;\">Unit\u00e9 Sp\u00e9cialis\u00e9e Appui \u00e0 la Recherche et\r\n Transfert Technologique (ARTT)<\/div>\r\n <\/div>\r\n\r\n <div style=\"display: flex; justify-content: space-between; align-items: end; flex-wrap: wrap; gap: 20px;\">\r\n <div>\r\n <h1 style=\"margin: 0; font-size: 32px; line-height: 1.2;\">Rapport d'Analyse N\u00b0 034-2025<\/h1>\r\n <p style=\"margin: 5px 0 0; color: #6E6C55;\">Code: 6-ER ARTT 02-01 | \u00c9dition: 02 | R\u00e9vision:\r\n 13\/03\/2025<\/p>\r\n <\/div>\r\n <div style=\"text-align: right;\">\r\n <div style=\"font-size: 14px; font-weight: 600; color: #3A3930;\">Dr. Soumaya Bourgou<\/div>\r\n <div style=\"font-size: 13px; color: #6E6C55;\">Laboratoire des Plantes Aromatiques et M\u00e9dicinales\r\n (LPAM)<\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n<\/header>\r\n\r\n<div class=\"container\" style=\"padding-top: 40px; padding-bottom: 80px;\">\r\n\r\n \r\n <div class=\"report-meta\">\r\n <div>\r\n <strong>Re\u00e7u le<\/strong>\r\n 18 septembre 2025\r\n <\/div>\r\n <div>\r\n <strong>Fin des analyses<\/strong>\r\n 19 septembre 2025\r\n <\/div>\r\n <div>\r\n <strong>Nature \u00e9chantillon<\/strong>\r\n Huile d'olive\r\n <\/div>\r\n <div>\r\n <strong>Nombre d'\u00e9chantillons<\/strong>\r\n 03 (T\u00e9moin, OZ, OX)\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <section>\r\n <h2 style=\"font-size: 24px; border-bottom: 1px solid #D6D3C0; padding-bottom: 10px; margin-bottom: 30px;\">\r\n 1. Analyse Physico-Chimique\r\n <\/h2>\r\n <p style=\"margin-bottom: 20px;\">\r\n <strong>M\u00e9thode utilis\u00e9e :<\/strong> M\u00e9thode \u00e0 froid. Sans purification avec silicagel SPE.<br>\r\n <em>NB : Les r\u00e9sultats du pr\u00e9sent Rapport d'Analyse ne se rapportent qu'aux \u00e9chantillons soumis \u00e0\r\n l'analyse.<\/em>\r\n <\/p>\r\n\r\n <div style=\"overflow-x: auto;\">\r\n <table class=\"scientific-table\">\r\n <thead>\r\n <tr>\r\n <th style=\"width: 25%;\">Param\u00e8tres analytiques<\/th>\r\n <th style=\"width: 20%;\">M\u00e9thodes d'essais<\/th>\r\n <th style=\"width: 15%;\">T\u00e9moin (Vierge)<\/th>\r\n <th style=\"width: 15%;\">OZ<\/th>\r\n <th style=\"width: 15%;\">OX (Hyper)<\/th>\r\n <th style=\"width: 10%;\">Crit\u00e8res (Vierge Extra)<\/th>\r\n <\/tr>\r\n <\/thead>\r\n <tbody>\r\n \r\n <tr>\r\n <td><strong>Acidit\u00e9 (%)<\/strong><\/td>\r\n <td>COI\/T.20\/Doc. N\u00b034 (2017)<\/td>\r\n <td>0.47<\/td>\r\n <td>0.25<\/td>\r\n <td>0.56<\/td>\r\n <td>\u2264 0.80<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>K232<\/strong><\/td>\r\n <td rowspan=\"3\">COI\/T.20\/Doc. N\u00b019 (2019)<\/td>\r\n <td>2.64<\/td>\r\n <td>2.65<\/td>\r\n <td>12.21<\/td>\r\n <td>\u2264 2.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>K270<\/strong><\/td>\r\n <td>0.16<\/td>\r\n <td>0.18<\/td>\r\n <td>0.17<\/td>\r\n <td>\u2264 0.22<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Delta K (AK)<\/strong><\/td>\r\n <td>0.000<\/td>\r\n <td>0.006<\/td>\r\n <td>-0.001<\/td>\r\n <td>\u2264 0.01<\/td>\r\n <\/tr>\r\n <tr class=\"highlight-row\">\r\n <td><strong>Indice de peroxyde (meq O2\/Kg)<\/strong><\/td>\r\n <td>COI\/T.20\/Doc. N\u00b035 (2017)<\/td>\r\n <td>16.2<\/td>\r\n <td>22.6<\/td>\r\n <td><strong>116.6<\/strong><\/td> \r\n <td>\u2264 20.0<\/td>\r\n <\/tr>\r\n\r\n \r\n <tr>\r\n <td colspan=\"6\"\r\n style=\"background-color: #F4F2E9; font-weight: 600; font-size: 12px; letter-spacing: 0.05em; text-transform: uppercase;\">\r\n Composition en Acides Gras (%) - COI\/T.20\/Doc. 33 (2017)<\/td>\r\n <\/tr>\r\n\r\n <tr>\r\n <td>Acide caprylique (C8:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.00<\/td>\r\n <td>0.02<\/td>\r\n <td>0.01<\/td>\r\n <td>-<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide Myristique (C14:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.01<\/td>\r\n <td>0.01<\/td>\r\n <td>0.02<\/td>\r\n <td>\u2264 0.03<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide palmitique (C16:0)<\/td>\r\n <td>-<\/td>\r\n <td>16.37<\/td>\r\n <td>13.54<\/td>\r\n <td>16.95<\/td>\r\n <td>7.00 - 20.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide palmitol\u00e9ique (C16:1)<\/td>\r\n <td>-<\/td>\r\n <td>1.64<\/td>\r\n <td>1.39<\/td>\r\n <td>1.65<\/td>\r\n <td>0.30 - 3.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide heptad\u00e9cano\u00efque (C17:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.04<\/td>\r\n <td>0.04<\/td>\r\n <td>0.04<\/td>\r\n <td>\u2264 0.40<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide heptad\u00e9c\u00e9no\u00efque (C17:1)<\/td>\r\n <td>-<\/td>\r\n <td>0.07<\/td>\r\n <td>0.08<\/td>\r\n <td>0.07<\/td>\r\n <td>\u2264 0.60<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide st\u00e9arique (C18:0)<\/td>\r\n <td>-<\/td>\r\n <td>2.43<\/td>\r\n <td>2.68<\/td>\r\n <td>2.53<\/td>\r\n <td>0.50 - 5.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide ol\u00e9ique (C18:1)<\/td>\r\n <td>-<\/td>\r\n <td>64.62<\/td>\r\n <td>71.97<\/td>\r\n <td>65.46<\/td>\r\n <td>55.00 - 85.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide linol\u00e9ique (C18:2)<\/td>\r\n <td>-<\/td>\r\n <td>13.39<\/td>\r\n <td>9.02<\/td>\r\n <td>11.92<\/td>\r\n <td>2.50 - 21.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide linol\u00e9nique (C18:3)<\/td>\r\n <td>-<\/td>\r\n <td>0.71<\/td>\r\n <td>0.59<\/td>\r\n <td>0.56<\/td>\r\n <td>\u2264 1.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide arachidique (C20:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.41<\/td>\r\n <td>0.36<\/td>\r\n <td>0.42<\/td>\r\n <td>\u2264 0.60<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide eicos\u00e9no\u00efque (C20:1)<\/td>\r\n <td>-<\/td>\r\n <td>0.18<\/td>\r\n <td>0.18<\/td>\r\n <td>0.20<\/td>\r\n <td>\u2264 0.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide b\u00e9h\u00e9nique (C22:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.11<\/td>\r\n <td>0.10<\/td>\r\n <td>0.13<\/td>\r\n <td>\u2264 0.20<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide lignoc\u00e9rique (C24:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.03<\/td>\r\n <td>0.04<\/td>\r\n <td>0.05<\/td>\r\n <td>\u2264 0.20<\/td>\r\n <\/tr>\r\n <\/tbody>\r\n <\/table>\r\n <\/div>\r\n\r\n <div\r\n style=\"display: flex; justify-content: space-between; margin-top: 40px; padding-top: 20px; border-top: 1px dashed #D6D3C0;\">\r\n <div>\r\n <strong>Dr Imen Oueslati<\/strong><br>\r\n <span style=\"font-size: 13px; color: #6E6C55;\">Cheffe de l'ARTT<\/span>\r\n <\/div>\r\n <div>\r\n <strong>Prof Chokri Messaoud<\/strong><br>\r\n <span style=\"font-size: 13px; color: #6E6C55;\">Directeur G\u00e9n\u00e9ral<\/span>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <div class=\"section-separator\"><\/div>\r\n\r\n \r\n <section>\r\n <div\r\n style=\"background: #FCFCFA; padding: 40px; border-radius: 8px; border: 1px solid #EAE8DC; text-align: center; margin-bottom: 50px;\">\r\n <h2 style=\"font-size: 28px; margin-bottom: 20px;\">Rapport Scientifique : Activit\u00e9s Biologiques<\/h2>\r\n <p style=\"font-size: 16px; color: #5F5E48; max-width: 800px; margin: 0 auto;\">\r\n \"Evaluation des activit\u00e9s biologiques d\u2019une huile d\u2019olive hyperoxyg\u00e9n\u00e9e : Analyse de l\u2019activit\u00e9\r\n antioxydante et de l\u2019effet cytotoxique et de l\u2019activit\u00e9 anti-inflammatoire sur lign\u00e9e cellulaire.\r\n Comparaison avec huile d\u2019olive vierge et huile d\u2019olive ozon\u00e9e.\"\r\n <\/p>\r\n <div style=\"margin-top: 20px; font-weight: 600; color: #84825A;\">Dr. Bourgou Soumaya \u2014 Septembre 2025\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 1. Etude de la cytotoxicit\u00e9 sur cellules saines\r\n <\/h3>\r\n\r\n <p><strong>Objectif :<\/strong> V\u00e9rifier que la mol\u00e9cule active n\u2019induit aucune modification de la\r\n viabilit\u00e9 cellulaire par rapport aux cellules non trait\u00e9es (macrophages).<\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>Mod\u00e8le cellulaire :<\/strong> Macrophages murins RAW 264.7 (ATCC).<\/li>\r\n <li><strong>Conditions de culture :<\/strong> Milleu RPMI 1640 s + 10% s\u00e9rum f\u0153tal bovin (FBS),\r\n 37\u00b0C, 5% CO2.<\/li>\r\n <li><strong>Densit\u00e9 d'ensemencement :<\/strong> 2 x 10<sup>5<\/sup> cellules\/puits (Plaques 24\r\n puits).<\/li>\r\n <li><strong>Test utilis\u00e9 :<\/strong> R\u00e9duction de la r\u00e9sazurine (indicateur m\u00e9tabolique\r\n mitochondrial).<\/li>\r\n <li><strong>Incubation :<\/strong> 24h d'adh\u00e9sion + 24h de traitement avec extraits.<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats (Viabilit\u00e9 Cellulaire)<\/h4>\r\n <p>Les r\u00e9sultats r\u00e9v\u00e8lent qu\u2019aucune toxicit\u00e9 n\u2019a \u00e9t\u00e9 d\u00e9tect\u00e9e dans l\u2019huile hyperoxyg\u00e9n\u00e9e ni dans\r\n l\u2019huile vierge.<\/p>\r\n\r\n <div\r\n style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 20px; margin-top: 20px;\">\r\n <div style=\"padding: 15px; background: #F4F9F4; border-radius: 6px;\">\r\n <span class=\"result-highlight\">> 90%<\/span>\r\n <span style=\"font-size: 13px;\">Viabilit\u00e9 pour toutes concentrations (50-400 \u00b5g\/mL)<\/span>\r\n <\/div>\r\n <div style=\"padding: 15px; background: #FFFCF0; border-radius: 6px;\">\r\n <span class=\"result-highlight\">+ 15%<\/span>\r\n <span style=\"font-size: 13px;\">Augmentation prolif\u00e9ration \u00e0 50 \u00b5g\/mL (Huile\r\n Hyperoxyg\u00e9n\u00e9e)<\/span>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 2. Etude de l'activit\u00e9 anti-inflammatoire\r\n <\/h3>\r\n\r\n <p><strong>Principe :<\/strong> Mesure de la capacit\u00e9 \u00e0 inhiber la production du monoxyde d\u2019azote (NO\u2022)\r\n par les macrophages stimul\u00e9s par une endotoxine (LPS). L'inhibition de NO contribue au traitement\r\n des pathologies inflammatoires.<\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>Pr\u00e9traitement :<\/strong> 1h avec concentrations croissantes d'extraits.<\/li>\r\n <li><strong>Stimulation :<\/strong> Ajout de LPS.<\/li>\r\n <li><strong>Incubation :<\/strong> 24h \u00e0 37\u00b0C.<\/li>\r\n <li><strong>Dosage :<\/strong> R\u00e9actif de GRIESS pour quantifier le NO produit.<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats d'Inhibition (Comparatif)<\/h4>\r\n <p>L\u2019huile hyperoxyg\u00e9n\u00e9e inhibe efficacement la production du radical NO, avec une activit\u00e9\r\n proportionnelle \u00e0 la dose et nettement sup\u00e9rieure \u00e0 l'huile vierge.<\/p>\r\n\r\n <table class=\"scientific-table\" style=\"margin-top: 20px; margin-bottom: 0;\">\r\n <thead>\r\n <tr>\r\n <th>Concentration<\/th>\r\n <th>Huile Hyperoxyg\u00e9n\u00e9e (Inhibition)<\/th>\r\n <th>Huile Vierge (Inhibition)<\/th>\r\n <th>Observation<\/th>\r\n <\/tr>\r\n <\/thead>\r\n <tbody>\r\n <tr>\r\n <td><strong>50 - 100 mg\/mL<\/strong><\/td>\r\n <td>~ 25%<\/td>\r\n <td>~ 7%<\/td>\r\n <td>Activit\u00e9 x3.5 sup\u00e9rieure<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>400 \u00b5g\/mL<\/strong><\/td>\r\n <td style=\"font-weight: 600; color: #84825A;\">33%<\/td>\r\n <td>14%<\/td>\r\n <td>Efficacit\u00e9 maximale observ\u00e9e<\/td>\r\n <\/tr>\r\n <\/tbody>\r\n <\/table>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 3. Etude de l\u2019activit\u00e9 antioxydante (DPPH)\r\n <\/h3>\r\n\r\n <p><strong>Principe :<\/strong> Test au radical synth\u00e9tique 2,2-diphenyl-1-picryle-hydrazyle (DPPH\u02d9). La\r\n d\u00e9coloration (du violet) refl\u00e8te le pouvoir de l\u2019extrait \u00e0 pi\u00e9ger ce radical via le don de protons.\r\n <\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>R\u00e9actifs :<\/strong> 1 mL d\u2019extrait + 250 \u03bcL de solution DPPH\u02d9 (0,2 mM).<\/li>\r\n <li><strong>Condition :<\/strong> Agitation vigoureuse + Repos \u00e0 l'obscurit\u00e9.<\/li>\r\n <li><strong>Mesure :<\/strong> Absorbance \u00e0 517 nm (compar\u00e9e au t\u00e9moin).<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats (% Inhibition DPPH)<\/h4>\r\n <div style=\"display: flex; gap: 40px; flex-wrap: wrap;\">\r\n <div style=\"flex: 1;\">\r\n <ul class=\"dashed\">\r\n <li><strong>\u00c0 50 mg\/mL :<\/strong> 40% d'inhibition.<\/li>\r\n <li><strong>\u00c0 100 mg\/mL :<\/strong> 70% d'inhibition.<\/li>\r\n <\/ul>\r\n <\/div>\r\n <div\r\n style=\"flex: 1; padding: 15px; background: #FBFBF6; border: 1px dashed #D6D3C0; border-radius: 4px; text-align: center;\">\r\n <div style=\"font-size: 12px; text-transform: uppercase;\">Valeur Cl\u00e9 CI50<\/div>\r\n <div style=\"font-size: 32px; font-family: 'Georgia', serif; color: #84825A;\">66 mg\/mL<\/div>\r\n <div style=\"font-size: 12px; color: #6E6C55;\">Concentration inhibitrice m\u00e9diane<\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div\r\n style=\"margin-top: 80px; padding: 40px; background: #5F5E48; color: #ffffff; border-radius: 4px; text-align: center;\">\r\n <h2\r\n style=\"color: #ffffff; font-size: 24px; margin-bottom: 20px; border-bottom: 1px solid rgba(255,255,255,0.2); padding-bottom: 15px; display: inline-block;\">\r\n Conclusion G\u00e9n\u00e9rale<\/h2>\r\n <p style=\"font-size: 18px; line-height: 1.8; margin: 0; font-family: 'Georgia', serif; font-style: italic;\">\r\n \"En conclusion, nos diff\u00e9rents r\u00e9sultats ont montr\u00e9 que l\u2019huile d\u2019olive hyperoxyg\u00e9n\u00e9e est dot\u00e9e\r\n d\u2019une bonne activit\u00e9 antioxydante et d\u2019une activit\u00e9 anti-inflammatoire appr\u00e9ciable sup\u00e9rieure \u00e0\r\n l\u2019huile d\u2019olive vierge. Ces potentialit\u00e9s sont associ\u00e9es \u00e0 une faible toxicit\u00e9 ex-vivo aux doses\r\n efficaces.\"\r\n <\/p>\r\n <div\r\n style=\"margin-top: 30px; font-size: 14px; opacity: 0.8; letter-spacing: 0.1em; text-transform: uppercase;\">\r\n Dr. Soumaya Bourgou \u2014 Laboratoire des Plantes Aromatiques et M\u00e9dicinales\r\n <\/div>\r\n <\/div>\r\n\r\n <\/section>\r\n\r\n<\/div>\r\n\r\n<\/div>\r\n<\/body>\r\n\r\n<\/html>\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Rapport d’Analyse Scientifique – Soresco Lab Centre de Biotechnologie de Borj-Cedria (CBBC) Unit\u00e9 Sp\u00e9cialis\u00e9e Appui \u00e0 la Recherche et Transfert Technologique (ARTT) Rapport d’Analyse N\u00b0 034-2025 Code: 6-ER ARTT 02-01 | \u00c9dition: 02 | R\u00e9vision: 13\/03\/2025 Dr. Soumaya Bourgou Laboratoire des Plantes Aromatiques et M\u00e9dicinales (LPAM) Re\u00e7u le 18 septembre 2025 Fin des analyses 19…<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2241","page","type-page","status-publish","hentry","description-off"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/comments?post=2241"}],"version-history":[{"count":7,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241\/revisions"}],"predecessor-version":[{"id":2544,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241\/revisions\/2544"}],"wp:attachment":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/media?parent=2241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

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\n\t\t\t\r\n\r\n\r\n\r\n \r\n \r\n Rapport d'Analyse Scientifique - Soresco Lab<\/title>\r\n <link rel=\"preconnect\" href=\"https:\/\/fonts.googleapis.com\">\r\n <link rel=\"preconnect\" href=\"https:\/\/fonts.gstatic.com\" crossorigin>\r\n <link\r\n href=\"https:\/\/fonts.googleapis.com\/css2?family=Inter:wght@300;400;500;600&family=Georgia:ital,wght@0,400;1,400&display=swap\"\r\n rel=\"stylesheet\">\r\n <style>\r\n <\/head><body>< !-- FULL WIDTH WRAPPER FOR WORDPRESS --><div style=\"width: 100vw; margin-left: calc(-50vw + 50%); position: relative; overflow-x: hidden; background: #fff;\">h1,\r\n h2,\r\n h3,\r\n h4,\r\n h5 {\r\n font-family: 'Georgia', serif;\r\n color: #3A3930;\r\n font-weight: 400;\r\n }\r\n\r\n .container {\r\n max-width: 95%;\r\n \/* Increased from 1000px for full width feel *\/\r\n margin: 0 auto;\r\n padding: 0 80px;\r\n \/* Extra padding left and right *\/\r\n }\r\n\r\n \/* HEADER *\/\r\n header {\r\n background-color: #fff;\r\n padding: 40px 0;\r\n border-bottom: 2px solid #F0EFE9;\r\n }\r\n\r\n .report-meta {\r\n font-size: 16px;\r\n \/* Increased meta font size *\/\r\n color: #6E6C55;\r\n display: grid;\r\n grid-template-columns: repeat(auto-fit, minmax(200px, 1fr));\r\n gap: 20px;\r\n margin-bottom: 40px;\r\n background: #FCFCFA;\r\n padding: 20px;\r\n border: 1px solid #EAE8DC;\r\n border-radius: 4px;\r\n }\r\n\r\n .report-meta strong {\r\n display: block;\r\n color: #3A3930;\r\n font-weight: 600;\r\n margin-bottom: 5px;\r\n }\r\n\r\n \/* TABLES *\/\r\n .scientific-table {\r\n width: 100%;\r\n border-collapse: collapse;\r\n font-size: 16px;\r\n \/* Increased table font size *\/\r\n margin-bottom: 40px;\r\n box-shadow: 0 2px 10px rgba(0, 0, 0, 0.03);\r\n }\r\n\r\n .scientific-table th,\r\n .scientific-table td {\r\n padding: 12px 15px;\r\n text-align: left;\r\n border: 1px solid #EAE8DC;\r\n }\r\n\r\n .scientific-table th {\r\n background-color: #F4F2E9;\r\n color: #3A3930;\r\n font-weight: 600;\r\n position: sticky;\r\n top: 0;\r\n }\r\n\r\n .scientific-table tr:nth-child(even) {\r\n background-color: #FCFCFA;\r\n }\r\n\r\n .scientific-table tr:hover {\r\n background-color: #F9F8F4;\r\n }\r\n\r\n .highlight-row {\r\n background-color: #FFFBE6 !important;\r\n }\r\n\r\n .section-separator {\r\n margin: 60px 0;\r\n height: 1px;\r\n background: #D4D1BC;\r\n opacity: 0.5;\r\n }\r\n\r\n \/* BIOLOGICAL STUDY *\/\r\n .bio-study-block {\r\n margin-bottom: 60px;\r\n }\r\n\r\n .protocol-box {\r\n background: #F9F8F4;\r\n padding: 30px;\r\n border-left: 4px solid #84825A;\r\n margin: 20px 0 30px;\r\n font-size: 16px;\r\n \/* Increased font size *\/\r\n }\r\n\r\n .results-box {\r\n background: #ffffff;\r\n border: 1px solid #EFECE1;\r\n padding: 30px;\r\n border-radius: 8px;\r\n }\r\n\r\n .result-highlight {\r\n font-size: 18px;\r\n color: #84825A;\r\n font-family: 'Georgia', serif;\r\n margin-bottom: 10px;\r\n display: block;\r\n }\r\n\r\n ul.dashed {\r\n list-style: none;\r\n padding-left: 0;\r\n }\r\n\r\n ul.dashed li {\r\n position: relative;\r\n padding-left: 20px;\r\n margin-bottom: 10px;\r\n }\r\n\r\n ul.dashed li::before {\r\n content: \"-\";\r\n position: absolute;\r\n left: 0;\r\n color: #84825A;\r\n }\r\n\r\n .print-only {\r\n display: none;\r\n }\r\n\r\n @media print {\r\n .print-only {\r\n display: block;\r\n }\r\n\r\n .no-print {\r\n display: none;\r\n }\r\n\r\n body {\r\n font-size: 12px;\r\n }\r\n\r\n .container {\r\n max-width: 100%;\r\n padding: 0;\r\n }\r\n }\r\n <\/style>\r\n<\/head>\r\n\r\n\r\n\r\n<header>\r\n <div class=\"container\">\r\n <div style=\"text-align: center; margin-bottom: 40px;\">\r\n <h4 style=\"margin: 0; font-size: 14px; letter-spacing: 0.2em; text-transform: uppercase; color: #84825A;\">\r\n Centre de Biotechnologie de Borj-Cedria (CBBC)<\/h4>\r\n <div style=\"font-size: 12px; margin-top: 5px; opacity: 0.7;\">Unit\u00e9 Sp\u00e9cialis\u00e9e Appui \u00e0 la Recherche et\r\n Transfert Technologique (ARTT)<\/div>\r\n <\/div>\r\n\r\n <div style=\"display: flex; justify-content: space-between; align-items: end; flex-wrap: wrap; gap: 20px;\">\r\n <div>\r\n <h1 style=\"margin: 0; font-size: 32px; line-height: 1.2;\">Rapport d'Analyse N\u00b0 034-2025<\/h1>\r\n <p style=\"margin: 5px 0 0; color: #6E6C55;\">Code: 6-ER ARTT 02-01 | \u00c9dition: 02 | R\u00e9vision:\r\n 13\/03\/2025<\/p>\r\n <\/div>\r\n <div style=\"text-align: right;\">\r\n <div style=\"font-size: 14px; font-weight: 600; color: #3A3930;\">Dr. Soumaya Bourgou<\/div>\r\n <div style=\"font-size: 13px; color: #6E6C55;\">Laboratoire des Plantes Aromatiques et M\u00e9dicinales\r\n (LPAM)<\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n<\/header>\r\n\r\n<div class=\"container\" style=\"padding-top: 40px; padding-bottom: 80px;\">\r\n\r\n \r\n <div class=\"report-meta\">\r\n <div>\r\n <strong>Re\u00e7u le<\/strong>\r\n 18 septembre 2025\r\n <\/div>\r\n <div>\r\n <strong>Fin des analyses<\/strong>\r\n 19 septembre 2025\r\n <\/div>\r\n <div>\r\n <strong>Nature \u00e9chantillon<\/strong>\r\n Huile d'olive\r\n <\/div>\r\n <div>\r\n <strong>Nombre d'\u00e9chantillons<\/strong>\r\n 03 (T\u00e9moin, OZ, OX)\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <section>\r\n <h2 style=\"font-size: 24px; border-bottom: 1px solid #D6D3C0; padding-bottom: 10px; margin-bottom: 30px;\">\r\n 1. Analyse Physico-Chimique\r\n <\/h2>\r\n <p style=\"margin-bottom: 20px;\">\r\n <strong>M\u00e9thode utilis\u00e9e :<\/strong> M\u00e9thode \u00e0 froid. Sans purification avec silicagel SPE.<br>\r\n <em>NB : Les r\u00e9sultats du pr\u00e9sent Rapport d'Analyse ne se rapportent qu'aux \u00e9chantillons soumis \u00e0\r\n l'analyse.<\/em>\r\n <\/p>\r\n\r\n <div style=\"overflow-x: auto;\">\r\n <table class=\"scientific-table\">\r\n <thead>\r\n <tr>\r\n <th style=\"width: 25%;\">Param\u00e8tres analytiques<\/th>\r\n <th style=\"width: 20%;\">M\u00e9thodes d'essais<\/th>\r\n <th style=\"width: 15%;\">T\u00e9moin (Vierge)<\/th>\r\n <th style=\"width: 15%;\">OZ<\/th>\r\n <th style=\"width: 15%;\">OX (Hyper)<\/th>\r\n <th style=\"width: 10%;\">Crit\u00e8res (Vierge Extra)<\/th>\r\n <\/tr>\r\n <\/thead>\r\n <tbody>\r\n \r\n <tr>\r\n <td><strong>Acidit\u00e9 (%)<\/strong><\/td>\r\n <td>COI\/T.20\/Doc. N\u00b034 (2017)<\/td>\r\n <td>0.47<\/td>\r\n <td>0.25<\/td>\r\n <td>0.56<\/td>\r\n <td>\u2264 0.80<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>K232<\/strong><\/td>\r\n <td rowspan=\"3\">COI\/T.20\/Doc. N\u00b019 (2019)<\/td>\r\n <td>2.64<\/td>\r\n <td>2.65<\/td>\r\n <td>12.21<\/td>\r\n <td>\u2264 2.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>K270<\/strong><\/td>\r\n <td>0.16<\/td>\r\n <td>0.18<\/td>\r\n <td>0.17<\/td>\r\n <td>\u2264 0.22<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Delta K (AK)<\/strong><\/td>\r\n <td>0.000<\/td>\r\n <td>0.006<\/td>\r\n <td>-0.001<\/td>\r\n <td>\u2264 0.01<\/td>\r\n <\/tr>\r\n <tr class=\"highlight-row\">\r\n <td><strong>Indice de peroxyde (meq O2\/Kg)<\/strong><\/td>\r\n <td>COI\/T.20\/Doc. N\u00b035 (2017)<\/td>\r\n <td>16.2<\/td>\r\n <td>22.6<\/td>\r\n <td><strong>116.6<\/strong><\/td> \r\n <td>\u2264 20.0<\/td>\r\n <\/tr>\r\n\r\n \r\n <tr>\r\n <td colspan=\"6\"\r\n style=\"background-color: #F4F2E9; font-weight: 600; font-size: 12px; letter-spacing: 0.05em; text-transform: uppercase;\">\r\n Composition en Acides Gras (%) - COI\/T.20\/Doc. 33 (2017)<\/td>\r\n <\/tr>\r\n\r\n <tr>\r\n <td>Acide caprylique (C8:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.00<\/td>\r\n <td>0.02<\/td>\r\n <td>0.01<\/td>\r\n <td>-<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide Myristique (C14:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.01<\/td>\r\n <td>0.01<\/td>\r\n <td>0.02<\/td>\r\n <td>\u2264 0.03<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide palmitique (C16:0)<\/td>\r\n <td>-<\/td>\r\n <td>16.37<\/td>\r\n <td>13.54<\/td>\r\n <td>16.95<\/td>\r\n <td>7.00 - 20.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide palmitol\u00e9ique (C16:1)<\/td>\r\n <td>-<\/td>\r\n <td>1.64<\/td>\r\n <td>1.39<\/td>\r\n <td>1.65<\/td>\r\n <td>0.30 - 3.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide heptad\u00e9cano\u00efque (C17:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.04<\/td>\r\n <td>0.04<\/td>\r\n <td>0.04<\/td>\r\n <td>\u2264 0.40<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide heptad\u00e9c\u00e9no\u00efque (C17:1)<\/td>\r\n <td>-<\/td>\r\n <td>0.07<\/td>\r\n <td>0.08<\/td>\r\n <td>0.07<\/td>\r\n <td>\u2264 0.60<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide st\u00e9arique (C18:0)<\/td>\r\n <td>-<\/td>\r\n <td>2.43<\/td>\r\n <td>2.68<\/td>\r\n <td>2.53<\/td>\r\n <td>0.50 - 5.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide ol\u00e9ique (C18:1)<\/td>\r\n <td>-<\/td>\r\n <td>64.62<\/td>\r\n <td>71.97<\/td>\r\n <td>65.46<\/td>\r\n <td>55.00 - 85.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide linol\u00e9ique (C18:2)<\/td>\r\n <td>-<\/td>\r\n <td>13.39<\/td>\r\n <td>9.02<\/td>\r\n <td>11.92<\/td>\r\n <td>2.50 - 21.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide linol\u00e9nique (C18:3)<\/td>\r\n <td>-<\/td>\r\n <td>0.71<\/td>\r\n <td>0.59<\/td>\r\n <td>0.56<\/td>\r\n <td>\u2264 1.00<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide arachidique (C20:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.41<\/td>\r\n <td>0.36<\/td>\r\n <td>0.42<\/td>\r\n <td>\u2264 0.60<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide eicos\u00e9no\u00efque (C20:1)<\/td>\r\n <td>-<\/td>\r\n <td>0.18<\/td>\r\n <td>0.18<\/td>\r\n <td>0.20<\/td>\r\n <td>\u2264 0.50<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide b\u00e9h\u00e9nique (C22:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.11<\/td>\r\n <td>0.10<\/td>\r\n <td>0.13<\/td>\r\n <td>\u2264 0.20<\/td>\r\n <\/tr>\r\n <tr>\r\n <td>Acide lignoc\u00e9rique (C24:0)<\/td>\r\n <td>-<\/td>\r\n <td>0.03<\/td>\r\n <td>0.04<\/td>\r\n <td>0.05<\/td>\r\n <td>\u2264 0.20<\/td>\r\n <\/tr>\r\n <\/tbody>\r\n <\/table>\r\n <\/div>\r\n\r\n <div\r\n style=\"display: flex; justify-content: space-between; margin-top: 40px; padding-top: 20px; border-top: 1px dashed #D6D3C0;\">\r\n <div>\r\n <strong>Dr Imen Oueslati<\/strong><br>\r\n <span style=\"font-size: 13px; color: #6E6C55;\">Cheffe de l'ARTT<\/span>\r\n <\/div>\r\n <div>\r\n <strong>Prof Chokri Messaoud<\/strong><br>\r\n <span style=\"font-size: 13px; color: #6E6C55;\">Directeur G\u00e9n\u00e9ral<\/span>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <div class=\"section-separator\"><\/div>\r\n\r\n \r\n <section>\r\n <div\r\n style=\"background: #FCFCFA; padding: 40px; border-radius: 8px; border: 1px solid #EAE8DC; text-align: center; margin-bottom: 50px;\">\r\n <h2 style=\"font-size: 28px; margin-bottom: 20px;\">Rapport Scientifique : Activit\u00e9s Biologiques<\/h2>\r\n <p style=\"font-size: 16px; color: #5F5E48; max-width: 800px; margin: 0 auto;\">\r\n \"Evaluation des activit\u00e9s biologiques d\u2019une huile d\u2019olive hyperoxyg\u00e9n\u00e9e : Analyse de l\u2019activit\u00e9\r\n antioxydante et de l\u2019effet cytotoxique et de l\u2019activit\u00e9 anti-inflammatoire sur lign\u00e9e cellulaire.\r\n Comparaison avec huile d\u2019olive vierge et huile d\u2019olive ozon\u00e9e.\"\r\n <\/p>\r\n <div style=\"margin-top: 20px; font-weight: 600; color: #84825A;\">Dr. Bourgou Soumaya \u2014 Septembre 2025\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 1. Etude de la cytotoxicit\u00e9 sur cellules saines\r\n <\/h3>\r\n\r\n <p><strong>Objectif :<\/strong> V\u00e9rifier que la mol\u00e9cule active n\u2019induit aucune modification de la\r\n viabilit\u00e9 cellulaire par rapport aux cellules non trait\u00e9es (macrophages).<\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>Mod\u00e8le cellulaire :<\/strong> Macrophages murins RAW 264.7 (ATCC).<\/li>\r\n <li><strong>Conditions de culture :<\/strong> Milleu RPMI 1640 s + 10% s\u00e9rum f\u0153tal bovin (FBS),\r\n 37\u00b0C, 5% CO2.<\/li>\r\n <li><strong>Densit\u00e9 d'ensemencement :<\/strong> 2 x 10<sup>5<\/sup> cellules\/puits (Plaques 24\r\n puits).<\/li>\r\n <li><strong>Test utilis\u00e9 :<\/strong> R\u00e9duction de la r\u00e9sazurine (indicateur m\u00e9tabolique\r\n mitochondrial).<\/li>\r\n <li><strong>Incubation :<\/strong> 24h d'adh\u00e9sion + 24h de traitement avec extraits.<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats (Viabilit\u00e9 Cellulaire)<\/h4>\r\n <p>Les r\u00e9sultats r\u00e9v\u00e8lent qu\u2019aucune toxicit\u00e9 n\u2019a \u00e9t\u00e9 d\u00e9tect\u00e9e dans l\u2019huile hyperoxyg\u00e9n\u00e9e ni dans\r\n l\u2019huile vierge.<\/p>\r\n\r\n <div\r\n style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 20px; margin-top: 20px;\">\r\n <div style=\"padding: 15px; background: #F4F9F4; border-radius: 6px;\">\r\n <span class=\"result-highlight\">> 90%<\/span>\r\n <span style=\"font-size: 13px;\">Viabilit\u00e9 pour toutes concentrations (50-400 \u00b5g\/mL)<\/span>\r\n <\/div>\r\n <div style=\"padding: 15px; background: #FFFCF0; border-radius: 6px;\">\r\n <span class=\"result-highlight\">+ 15%<\/span>\r\n <span style=\"font-size: 13px;\">Augmentation prolif\u00e9ration \u00e0 50 \u00b5g\/mL (Huile\r\n Hyperoxyg\u00e9n\u00e9e)<\/span>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 2. Etude de l'activit\u00e9 anti-inflammatoire\r\n <\/h3>\r\n\r\n <p><strong>Principe :<\/strong> Mesure de la capacit\u00e9 \u00e0 inhiber la production du monoxyde d\u2019azote (NO\u2022)\r\n par les macrophages stimul\u00e9s par une endotoxine (LPS). L'inhibition de NO contribue au traitement\r\n des pathologies inflammatoires.<\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>Pr\u00e9traitement :<\/strong> 1h avec concentrations croissantes d'extraits.<\/li>\r\n <li><strong>Stimulation :<\/strong> Ajout de LPS.<\/li>\r\n <li><strong>Incubation :<\/strong> 24h \u00e0 37\u00b0C.<\/li>\r\n <li><strong>Dosage :<\/strong> R\u00e9actif de GRIESS pour quantifier le NO produit.<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats d'Inhibition (Comparatif)<\/h4>\r\n <p>L\u2019huile hyperoxyg\u00e9n\u00e9e inhibe efficacement la production du radical NO, avec une activit\u00e9\r\n proportionnelle \u00e0 la dose et nettement sup\u00e9rieure \u00e0 l'huile vierge.<\/p>\r\n\r\n <table class=\"scientific-table\" style=\"margin-top: 20px; margin-bottom: 0;\">\r\n <thead>\r\n <tr>\r\n <th>Concentration<\/th>\r\n <th>Huile Hyperoxyg\u00e9n\u00e9e (Inhibition)<\/th>\r\n <th>Huile Vierge (Inhibition)<\/th>\r\n <th>Observation<\/th>\r\n <\/tr>\r\n <\/thead>\r\n <tbody>\r\n <tr>\r\n <td><strong>50 - 100 mg\/mL<\/strong><\/td>\r\n <td>~ 25%<\/td>\r\n <td>~ 7%<\/td>\r\n <td>Activit\u00e9 x3.5 sup\u00e9rieure<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>400 \u00b5g\/mL<\/strong><\/td>\r\n <td style=\"font-weight: 600; color: #84825A;\">33%<\/td>\r\n <td>14%<\/td>\r\n <td>Efficacit\u00e9 maximale observ\u00e9e<\/td>\r\n <\/tr>\r\n <\/tbody>\r\n <\/table>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div class=\"bio-study-block\">\r\n <h3\r\n style=\"font-size: 20px; color: #3A3930; border-left: 4px solid #D6D3C0; padding-left: 15px; margin-bottom: 20px;\">\r\n 3. Etude de l\u2019activit\u00e9 antioxydante (DPPH)\r\n <\/h3>\r\n\r\n <p><strong>Principe :<\/strong> Test au radical synth\u00e9tique 2,2-diphenyl-1-picryle-hydrazyle (DPPH\u02d9). La\r\n d\u00e9coloration (du violet) refl\u00e8te le pouvoir de l\u2019extrait \u00e0 pi\u00e9ger ce radical via le don de protons.\r\n <\/p>\r\n\r\n <div class=\"protocol-box\">\r\n <h5 style=\"margin: 0 0 10px;\">Protocole & Mode Op\u00e9ratoire<\/h5>\r\n <ul class=\"dashed\">\r\n <li><strong>R\u00e9actifs :<\/strong> 1 mL d\u2019extrait + 250 \u03bcL de solution DPPH\u02d9 (0,2 mM).<\/li>\r\n <li><strong>Condition :<\/strong> Agitation vigoureuse + Repos \u00e0 l'obscurit\u00e9.<\/li>\r\n <li><strong>Mesure :<\/strong> Absorbance \u00e0 517 nm (compar\u00e9e au t\u00e9moin).<\/li>\r\n <\/ul>\r\n <\/div>\r\n\r\n <div class=\"results-box\">\r\n <h4 style=\"margin-top: 0;\">R\u00e9sultats (% Inhibition DPPH)<\/h4>\r\n <div style=\"display: flex; gap: 40px; flex-wrap: wrap;\">\r\n <div style=\"flex: 1;\">\r\n <ul class=\"dashed\">\r\n <li><strong>\u00c0 50 mg\/mL :<\/strong> 40% d'inhibition.<\/li>\r\n <li><strong>\u00c0 100 mg\/mL :<\/strong> 70% d'inhibition.<\/li>\r\n <\/ul>\r\n <\/div>\r\n <div\r\n style=\"flex: 1; padding: 15px; background: #FBFBF6; border: 1px dashed #D6D3C0; border-radius: 4px; text-align: center;\">\r\n <div style=\"font-size: 12px; text-transform: uppercase;\">Valeur Cl\u00e9 CI50<\/div>\r\n <div style=\"font-size: 32px; font-family: 'Georgia', serif; color: #84825A;\">66 mg\/mL<\/div>\r\n <div style=\"font-size: 12px; color: #6E6C55;\">Concentration inhibitrice m\u00e9diane<\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n\r\n \r\n <div\r\n style=\"margin-top: 80px; padding: 40px; background: #5F5E48; color: #ffffff; border-radius: 4px; text-align: center;\">\r\n <h2\r\n style=\"color: #ffffff; font-size: 24px; margin-bottom: 20px; border-bottom: 1px solid rgba(255,255,255,0.2); padding-bottom: 15px; display: inline-block;\">\r\n Conclusion G\u00e9n\u00e9rale<\/h2>\r\n <p style=\"font-size: 18px; line-height: 1.8; margin: 0; font-family: 'Georgia', serif; font-style: italic;\">\r\n \"En conclusion, nos diff\u00e9rents r\u00e9sultats ont montr\u00e9 que l\u2019huile d\u2019olive hyperoxyg\u00e9n\u00e9e est dot\u00e9e\r\n d\u2019une bonne activit\u00e9 antioxydante et d\u2019une activit\u00e9 anti-inflammatoire appr\u00e9ciable sup\u00e9rieure \u00e0\r\n l\u2019huile d\u2019olive vierge. Ces potentialit\u00e9s sont associ\u00e9es \u00e0 une faible toxicit\u00e9 ex-vivo aux doses\r\n efficaces.\"\r\n <\/p>\r\n <div\r\n style=\"margin-top: 30px; font-size: 14px; opacity: 0.8; letter-spacing: 0.1em; text-transform: uppercase;\">\r\n Dr. Soumaya Bourgou \u2014 Laboratoire des Plantes Aromatiques et M\u00e9dicinales\r\n <\/div>\r\n <\/div>\r\n\r\n <\/section>\r\n\r\n<\/div>\r\n\r\n<\/div>\r\n<\/body>\r\n\r\n<\/html>\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Rapport d’Analyse Scientifique – Soresco Lab Centre de Biotechnologie de Borj-Cedria (CBBC) Unit\u00e9 Sp\u00e9cialis\u00e9e Appui \u00e0 la Recherche et Transfert Technologique (ARTT) Rapport d’Analyse N\u00b0 034-2025 Code: 6-ER ARTT 02-01 | \u00c9dition: 02 | R\u00e9vision: 13\/03\/2025 Dr. Soumaya Bourgou Laboratoire des Plantes Aromatiques et M\u00e9dicinales (LPAM) Re\u00e7u le 18 septembre 2025 Fin des analyses 19…<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2241","page","type-page","status-publish","hentry","description-off"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/comments?post=2241"}],"version-history":[{"count":7,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241\/revisions"}],"predecessor-version":[{"id":2544,"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/pages\/2241\/revisions\/2544"}],"wp:attachment":[{"href":"https:\/\/sorescolab.com\/en\/wp-json\/wp\/v2\/media?parent=2241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}